Studies on Subunits of Human Chorionic Gonadotropin
نویسندگان
چکیده
منابع مشابه
Immunoassay of human chorionic gonadotropin, its free subunits, and metabolites.
Multiple hCG-related molecules are present in pregnancy serum and urine samples. These include non-nicked hCG (the hormone), nicked hCG, hyper- and hypoglycosylated hCG, hCG missing the C-terminal extension, free alpha-subunit, large free alpha-subunit, free beta-subunit, nicked free beta-subunit, and beta-core fragment. Over 100 immunoassays are sold for quantifying hCG-related molecules in se...
متن کامل[Purification of subunits from human chorionic gonadotropin and radioimmunoassay].
A crude hCG with an activity of about 3,000 IU per mg was purifed to 10,000--15,000 IU per mg of dry weight using Amberlite CG-50 chromatography combined with DEAE-Sephadex A-50 and Sephadex G-75. The alpha and beta subunits of hCG were prepared by urea-treatment of the hormone and isolated by DEAE-Sephadex A-50 chromatography. Further purification of the subunits was achieved by gel filtration...
متن کاملHuman Chorionic Gonadotropin
A highly active preparation of human chorionic gonadotropin has been obtained by simple methods of column chromatography on diethylaminoethyl Sephadex and Sephadex G-100. The preparation appeared homogeneous by disc and immunoelectrophoresis, ultracentrifugation, and end group analysis. It assayed at 12,000 i.u. per mg by the rat prostrate gland and accessory sex organ method. The ~20,~ value w...
متن کاملImmunological studies with a commercial preparation of human chorionic gonadotropin.
Immunological methods are being used to determine human chorionic gonadotropin (HCG) in urine and serum during pregnancy (1-4) and also to localize it in tissues and tumors (5). The antisera used in these studies (1-5) were produced by immunizing rabbits with HCG preparations of widely varying specific activities. The HCG-HCG antisera characterization studies that were performed primarily with ...
متن کاملQuantification of Human Chorionic Gonadotropin by Bovine Serum Albumin Nanoparticles
Background and Aims: Some nanoparticles can be used in immunoassays to increase sensitivity. This study aimed to evaluate a novel nano-immunoassay based on bovine serum albumin nanoparticles (BSA NPs). Materials and methods: At first, the nanostructure was synthesized, and then applied as a tag in the nano-immunoassay. Then the concentration of β-subunit of human chorionic gonadotropin ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Folia Endocrinologica Japonica
سال: 1973
ISSN: 0029-0661
DOI: 10.1507/endocrine1927.49.3_599